Nitro Blue Tetrazolium Test
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The reduction of nitroblue tetrazolium nbt dye by neutrophils from 379 patients with infectious diseases and 268 controls has been examined.
Nitro blue tetrazolium test. The nitroblue tetrazolium test checks if certain immune system cells can change a colorless chemical called nitroblue tetrazolium nbt into a deep blue color. Nitroblue tetrazolium n b t is an interesting compound which is of value in the study of neutrophil physiology. How the test is performed.
The reaction can be monitored spectrophotometrically or the stained cells can be scored histochemically. When there is an nadph oxidase defect the phagocyte is unable to make reactive oxygen species or radicals required for bacterial killing. Nitro blue tetrazolium is used in a diagnostic test particularly for chronic granulomatous disease and other diseases of phagocyte function.
Chronic granulomatous disease cgd in utero has recently been detected by a new qualitative nitro blue tetrazolium nbt reduction slide test using phorbol myristate acetate pma as a stimulus. It is useful in the study of phagocytosis and factors effecting phagocytosis since its addition to blood results in the formation of a particulate object of phagocytosis while at the same time the dye which is complexed to this precipitate functions as a visible marker of the completion of this act. The mean nbt score was 29 8 72 3 positive tests in the 231 patients with non tuberculous bacterial infections 9 7 28 1 positive tests in the 135 patients with viral infections 5 3 1 5 positive tests in the controls.
Nbt will undergo re duction to form an insoluble blue black product formazan. As a result bacteria may thrive within the phagocyte. Stand the fundamental chemistry of the nitroblue tetrazolium nbt reaction that lies at the heart of this analysis.
The chemical nbt is added to the white blood cells in the lab. A blood sample is needed. The chemical nbt is added to the white blood cells in the lab.
The technique is simple and inexpensive and requires only a few microlitres of blood. How the test is performed a blood sample is needed.
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